AFI may refer to:
- American Film Institute The American Film Institute is an independent non-profit organization created by the National Endowment for the Arts, which was established in 1967 when President Lyndon B. Johnson signed the National Foundation on the Arts and the Humanities Act. The organization describes itself as "a national institute providing leadership in screen, an independent non-profit film organization
- Australian Film Institute The Australian Film Institute , established in 1958, is an organisation that promotes Australian film and television through the annual AFI Awards, a membership program and AFI film events throughout the year, an organisation that promotes Australian film and television
- AFI (band), an American rock band
- AFI (album), a 2004 album by AFI
- Agencia Federal de Investigación, a Mexican agency intended to fight corruption and organized crime
- Air Force Instruction (AFI), documented instructions for members of the United States Air Force
- American Football Israel, a nonprofit sports organization
- Amniotic fluid index, a measure of the amount of amniotic fluid, which is an index for fetal well being
- Aquarium Fish International Aquarium Fish International is a North American monthly magazine, published by BowTie Inc. of Irvine, California, and dedicated to freshwater and saltwater fishkeeping and the aquarium/fishkeeping hobby in general. Originally titled Aquarium Fish Magazine (AFM), the first issue of the magazine was in October 1988. The title of the publication, a North American monthly magazine
- Armed Forces Insurance, member-owned, non-profit insurance company for military professionals, headquartered in Leavenworth, KS
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